产品货号：
GS4489
中文名称：
重组人M-CSF
英文名称：
Recombinant Human Macrophage Colony Stimulating Factor
产品规格：
2μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 36.8kDa,a disulfide-linked homodimer consisting of two 158 amino acid polypeptide chains.
储存条件：冷冻（-20℃）
概述
Macrophage Colony Stimulating Factor(M-CSF),also named CSF-1,is a hematopoietic growth factor that is involved in the proliferation,differentiation,and survival of monocytes,macrophages,and bone marrow progenitor cells.It is produced by osteoblasts(as a result of endocrine stimulation by parathyroid hormone)exerts paracrine effects on osteoclasts and can interact with CSF1R.M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer.Four transcript variants encoding three different isoforms have been reported for M-CSF gene.Although forms may vary,all of them contain the N-terminal 150 a.a.portion that is necessary and sufficient for interaction with the receptor.The first 223 a.a.of mature human M-CSF shares 88%,86%,81%and 74%sequence identity with corresponding regions of dog,cow,mouse and rat M-CSF,respectively.Human M-CSF is active in the mouse,but mouse M-CSF is reported to be species-specific.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人M-CSF，M-CSF, Human
储存条件：冷冻（-20℃）
概述
Macrophage Colony Stimulating Factor(M-CSF),also named CSF-1,is a hematopoietic growth factor that is involved in the proliferation,differentiation,and survival of monocytes,macrophages,and bone marrow progenitor cells.It is produced by osteoblasts(as a result of endocrine stimulation by parathyroid hormone)exerts paracrine effects on osteoclasts and can interact with CSF1R.M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer.Four transcript variants encoding three different isoforms have been reported for M-CSF gene.Although forms may vary,all of them contain the N-terminal 150 a.a.portion that is necessary and sufficient for interaction with the receptor.The first 223 a.a.of mature human M-CSF shares 88%,86%,81%and 74%sequence identity with corresponding regions of dog,cow,mouse and rat M-CSF,respectively.Human M-CSF is active in the mouse,but mouse M-CSF is reported to be species-specific.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 1ng/ml,corresponding to a specific activity of>1.0×106 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered solution in PBS,pH7.4. |
| 内毒素 | Less than 1 EU/μg of rHuM-CSF as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water to a concentration of 0.1mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS |
相关搜索：重组人M-CSF，M-CSF, Human